when looking at data obtained from studies with living mammalian cells, it appears reasonable to assume that dequalinium molecules could be pulled into the mitochondrial matrix in response to the high mitochondrial membrane potential as demonstrated in , which in turn might lead to the destabilization of the dqasomepdna complex however, the first detailed study, which demonstrated the selective dna convert testosterone to estrogen release from dqaplexes, was performed using membrane mimicking liposomes fig as a model for the intracellular release of dna from dqasomes, the capacity of anionic liposomes to displace the dna convert testosterone to estrogen from its cationic carrier was studied the association of dna with the cationic carrier was assessed by employing sybr green i the fluorescence signal of this dye is greatly enhanced when bound to dna nonbinding results in loss of fluorescence it can be clearly seen that convert testosterone to estrogen in the vicinity of a charge ratio, dqasomes do not release any dna in the presence of cytoplasmic membrane mimicking liposomes dqasomes s � � o � u a � � � cpm imm ��� anionic liposomes i � � time [sec] fig effect of anionic liposomes on dna release from dqasomepdna complexes dna was preincubated with sybr until stabilization of the signal, followed by adding indicated by arrow the minimal amount of dqasomes necessary to decrease the signal to background level anionic liposomes were then injected arrow at an anionic to cationic charge ratio as shown the displacement of dna from its carrier is indicated by the increase of convert testosterone to estrogen the fluorescence signal cpm, cytoplasma membrane like liposomes imm, inner mitochondrial membrane like liposomes omm, outer mitochondrial membrane like liposomes cpm, not even at a fold excess of anionic charge however, with a similar charge excess of anionic liposomes to cationic dqasomes, and respectively, inner and outer mitochondrial membrane mimicking liposomes imm and omm, respectively are able to displace up to of the dna from its dqasomal carrier in agreement with these data, it was found that for the complete liberation of dna from dna dqasome complex, a fourfold excess of dicetylphosphate and an eightfold excess of phosphatidylserine, respectively, are necessary convert testosterone to estrogen the finding that cpm liposomes, at an anionic to cationic convert testosterone to estrogen charge ratio of , displace up to of the dna from lipofectin, which was used as convert testosterone to estrogen a control, do not liberate any dna from dqasomes even at a slight excess of anionic charge, leads to the conclusion convert testosterone to estrogen that besides the charge ratio, other factors may play an important role in the mechanism convert testosterone to estrogen of dna release from lipiddna convert testosterone to estrogen complexes this conclusion is being further supported by xu and szokas observation that ionic water soluble molecules such as atp, trna, dna, polyglutamic acid, spermidine and histone do not displace convert amiloride hctz 5mg testosterone to estrogen dna from the cationic lipiddna convert testosterone to estrogen complex, even at a fold charge excess � in their model for the postendocytotic release convert testosterone to estrogen of dna from cationic carriers, they assume the formation of a charge neutral ion pair convert testosterone to estrogen between cationic and anionic lipid, which ultimately results in the convert testosterone to estrogen displacement of the dna from the cationic lipid and the release of dna into cytoplasm liposomedna liposome ?