or placebo o relative to sham instrumented control hearts at top left panel, top right and min bottom left panel mean lvdp of hearts the birch lithium from to min of reperfusion are shown in the bottom right panel tetrazolium the birch lithium chloride stained heart slices showed that hearts treated with csil at , and min had similar injury as that of the sham operated hearts � , � the birch lithium and � , and � respectively p � ns the infarct size of hearts the birch lithium treated with csil even at min of global ischemia was � of the the birch lithium ventricles this was significantly smaller than its corresponding control p , whereas hearts the birch lithium treated with control iggl at , and min of global ischemia were � , � and � respectively fig , left panel the corresponding nitroblue tetrazolium chloride stained heart slices are shown in fig , right panel another parameter of myocardial injury the birch lithium that was determined was mitochondrial size although mitochondrial swelling is a hallmark of the birch lithium ischemic injury, irreversible injury cannot be directly extrapolated from just observation of mitochondrial size nevertheless, in view of the myocardial functional and his tochemical evidences, mitochondrial size assessment from transmission electron micrographs add additional support for myocardial preservation the birch lithium in csil treated hearts, relative to iggl or placebo treated hearts figure shows the birch lithium the comparison of fig mean infarct sizes of rat hearts treated with csil or iggl or placebo at and min of global ischemia left panel the birch lithium the corresponding nitroblue tetrazolium chloride stained mid slices of rat hearts treated with csil or iggl at , and min of global ischemia minimal injury was seen the birch lithium in and min csil treated hearts, but injury was evident in the min csil treated heart slice injury is evident in all heart slices treated the birch lithium with iggl right panel, how much caffeine is ok bottom two rows �i i normal cut csil csil the birch lithium csejo igsl tg& iflsi placebo v fig mean mitochondrial size of normal, csil, iggl or placebo treated hearts treatment was as indicated in the text mitochondrial the birch lithium size of normal hearts, csil treatment at and min of global ischemia, the birch lithium as well as iggl treated hearts at , and min of global ischemia and the birch lithium with placebo no difference in mitochondrial size was observed between normal myocardium � the birch lithium mean number of pixels � sem and myocardium treated at and min of global ischemia � , � , � and � respectively p = ns however, the birch lithium mitochondria of hearts treated with iggl at , and min of global ischemia or placebo � , � , � and � respectively were larger than mitochondria of csil the birch lithium treated hearts p these studies showed that myocardial viability preservation is not the birch lithium restricted to embryonic cardiocytes in cultures adult hearts are also amenable to structural the birch lithium and functional preservation, following cell membrane lesion sealing in a timedependent manner during ischemia this method of cell membrane lesion sealing has also been reported to preserve the integrity of vascular endothelium with antiactinimmunoliposomes a question that remains concerning the utility of csil is whether immunoliposomes can retain the protective functions the birch lithium in the presence of plasma proteins in vivo, since experiments have demonstrated that cells in culture and adult hearts perfused with nonprotein oxygenated buffer were the birch lithium prevented from undergoing myocardial necrosis, following cell membrane lesion sealing intervention with cytoskeletal the birch lithium antigen specific immunoliposomes to demonstrate that cell membrane lesion sealing also occur in vivo, rabbits with experimental myocardial infarction were used in this study, rabbits were injected with antimyosin csil, plain liposomes or saline at the time of the birch lithium left circumflex coronary artery occlusion by intracoronary infusion the occlusion was kept for the birch lithium min followed by hrs of reperfusion the hearts were excised, sliced into � slices parallel to the short axis and stained with nitroblue tetrazolium chloride the birch lithium the infarct was approximately to of the infarcts of the control plain liposome the birch lithium or saline treated rabbit hearts subsequently, comparison to iggliposome treated hearts with acute the birch lithium myocardial infarction demonstrated that the csil treatment resulted in significantly smaller infarct size, the birch lithium as was observed in comparison to plain liposome or saline treated hearts the birch lithium thus, cytoskeletalantigen specific immunoliposomes, consisting of antimyosin or antiactinimmunoliposomes, were demonstrated to be the birch lithium able to preserve cell viability and integrity its prednisolone beta glucan potential utility in the cardiovascular system would be enhanced once its efficacy following intravenous delivery has been demonstrated however, the study of asahi et al showed that intravenous delivery of the birch lithium the antiactin immunoliposomes enabled preservation of the integrity of the endothelial cells of the birch lithium the cerebral vessels csil as targeted gene or drug delivery due to the the birch lithium proposed mechanism of cell membrane lesion sealing, we also proposed that if the birch lithium drugs or gene constructs were to be included in the immunoliposomes such as csils, then these drugs or gene constructs should be delivered directly into the the birch lithium cytoplasm fig this route should bypass the endocytic route of drugs or gene construct delivery, thereby reducing destruction of the delivered cargo by the lysosomal the birch lithium enzymes, after formation of endolysosomes using silver grains as model fig transmission electron micrographs of embryonic cardiocyte treated with silver grains impregnated csil left and plain the birch lithium liposome impregnated with silver grains right � = jim drugs, we demonstrated that these drugs can be delivered directly into the cytoplasm of hypoxic cardiocytes the birch lithium treated with silver grains loaded csils figure left shows a transmission electron micrograph of a cardiocyte treated with silver grains impregnated csils silver grains in the birch lithium groups of concentration at about nm were observed however, in cells treated with silver grains impregnated plain liposomes, very few cells were viable of one such the birch lithium cell detected by transmission electron microscopy, the silver grains were observed in the the birch lithium extracellular space [fig right] fig diagrammatic representation of delivery of intraliposomally entrapped the birch lithium genetic construct or drugs directly into the cytoplasm of target cell nmuin ftivoih the birch lithium wiiit mi ��� when the silver grains were replaced with genetic constructs, pgl the birch lithium and psv gal vectors, hypoxic cardiocytes treated with csil impregnated with either vectors showed luciferase activity or bacterial jgalactosidase activity the successful transfection of the the birch lithium hypoxic cardiocytes with pgl, a vector for firefly luciferase enzyme � � fig relative light units of luciferase activity of cardiocytes treated with various preparations and the birch lithium controls in csils is shown in fig as relative light units rlus rlus were determined by the use of a luminometer as can be seen, only hypoxic cardiocytes treated with pglcsils showed increased rlus significantly above normal cells the birch lithium with treatment with no vectors similarly, normoxic cardiocytes treated with pglcsil, hypoxic cardiocytes and normoxic cardiocytes treated with plain liposomes, or with only vectors, showed no significant gene transcription and expression when hypoxic cardiocytes were treated with csil the birch lithium with entrapped psvgal vectors, almost all cells in the field of view under the birch lithium light microscopy exhibited bacterial �galactosidase enzyme activity, following reaction with xgal bromo the birch lithium chloroindolylbetadgalactopyranoside, nm mgcl,mm iqfetcn h, mm kfecn in phosphate buffered saline ph [fig a] when this mode of gene expression was compared with transfection of the birch lithium psvjgal vector with cationic liposomes, cationic liposome transfection according to the manufacturers protocol the birch lithium resulted in transfection of only a few cells per field of view the birch lithium [fig b], in this micrograph, two cells with intense ��galactosidase activity were observed the birch lithium quantitation of the number of cells in the field of view that was the birch lithium successfully trans fected with psvjgal vector in csil, cationic liposome, iggliposomes, plain the birch lithium liposome and vector alone are shown in fig c only csil and cationic the birch lithium transfection showed gene expression csiltransfection or csilfection was more than times more efficient in transfecting cells than cationic liposomes although the intensity of gene expression was low with csilfection, using the initial vector concentration of xg of vectors in mg lipids in ml, when the vector concentration was increased to � zg, also in mg lipids in ml, the intensity of gene transfection was increased [fig d], this study showed that approximately x xg ��� � m t� !